Annelids

 

 

In situ hybridization: principle and steps

 

In situ hybridization (ISH) is the specific annealing of a labeled RNA probe to complementary sequence of a target mRNA in a fixed specimen, followed by detection and visualization of the nucleic acid hybrids. By allowing detection of mRNA in tissues and in whole embryos, this technique enables to get a complete overview on the specific expression patterns of those genes (mRNA) in the course of embryonic and post-embryonic developments (from Baumgart et al., 2001).

 

 

 

 

 

 

Whole-body gene expression pattern registration:

 

This technique allows the rigorous comparison of expression patterns, at a specific developmental stage, of an animal which harbors invariant development. It requires a standardized reference template at the desired developmental stage and allows the production of a standardized digital anatomical atlases of gene expression patterns, at the near-cellular resolution (from Asadulina et al., 2012).